Figure: Determination of redox potential before and after fermentation of corn stover hydrolysate (A and B), determination of redox potential of electron carrier humic acid and neutral red in P2 medium (C and D) The development of lignocellulose-based liquid biofuels is in line with our country's biofuels policy of “not competing with food and not competing with peopleâ€. Corn stalk is a large class of representative lignocellulosic raw materials produced in agricultural production in China. It is widely distributed and produces large quantities. Improper handling can easily lead to environmental pollution. Bio-transformation of corn stalks to produce butanol is a turn-off process for waste, and it is a multi-purpose process. In the direction. In the process of bio-fermentation using corn stalk as raw material, the pre-treatment of corn stalks causes the lignin to degrade and produce a series of structurally different and soluble compounds called soluble lignin (Soluble lignin compounds). Soluble lignin will inhibit the downstream microbial fermentation process, significantly changing the proportion of fermentation products. Although scientists have conducted a lot of research on this inhibition, the specific mechanism is not clear. The microbial resources team of the Qingdao Institute of Bioenergy and Bioprocess Research, Chinese Academy of Sciences carried out an in-depth study of the effect of corn straw hydrolysate on Clostridium butyricum, and found that the corn straw hydrolysate can inhibit the growth and sugar utilization of Clostridium, and this inhibition can be achieved through hydrogen. The calcium oxide detoxification method removes. Soluble lignin in the hydrolysate can generate oxidative stress. Clostridium butyricum produces reduced NADH in the process of removing oxidative stress, which causes a change in the ratio of acetone to butanol in the final fermentation product, making acetone The ratio in the total solvent increases significantly. In the process of microbial fermentation, soluble lignin in the hydrolysate can provide electrons and promote the production of hydrogen gas from Clostridium butyricum. The above results provide important references for the study of butanol fermentation with lignocellulose as raw material in the future. The research results are published online in the journal Applied Environmental Microbiology. This work was done in collaboration with the Institute's Marine Carbon Group, Northeastern University and Sinopec Fushun Research Institute. The above research was supported by the National Natural Science Foundation of China, Shandong Province Jie Qing, the Director Innovation Fund, and Sinopec cooperation projects. Gateball Astro Turf, Gateball Grass, China Gateball Astro Turf JIANGSU WMGRASS CO., LTD. , https://www.wmgrassturf.com